Basic study for dinical application of a novel biomedical material "Anodized titanium plate

在这项研究中，我们研究了钛板的抗菌效果，阳极氧化的几种解决方案对致龋革兰氏阳性菌变形链球菌。选用NaCl、Na_2CO_3、MgSO_4、KCl、MgCl_2、CH_3COOK作为阳极氧化液。Na_2CO_3、MgSO_4、CH_3COOK无抑菌作用，而NaCl、KCl、MgCl_2有抑菌作用，卤素如Cl对抑菌作用是绝对必要的。对变形链球菌、戈登链球菌、远缘链球菌、金黄色葡萄球菌、化脓链球菌、白色念珠菌、光滑念珠菌的抑菌试验表明，对革兰氏阳性菌和革兰氏阴性菌均有较好的抑菌效果，但对真菌如白色念珠菌的抑菌效果较差。在无菌水中浸泡2周后，未观察到阳极氧化钛板的抗菌效果降低，然而，在磷酸盐缓冲盐水（PBS）或RPMI 1640培养基中浸泡后，抗菌效果显著降低。这些结果表明，抗菌效果是基于pH值。这可能是合理的，一些含氯的酸，如HClO从阳极氧化钛板表面释放。这些结果表明，这种新型的氯化钠阳极氧化钛板是有效的，以防止细菌的附着，并有效地附着宿主细胞，如成骨细胞。这种处置对于植入物使用可能非常有效，因为在这种情况下，我们不必重复使用这种材料。通过NaCl阳极氧化的植入物可能有效降低口腔和/或一般细菌感染的机会。

In this study, we examined the anti-bacterial effect of titanium plates, anodized by several solutions on cariogenic gram positive bacteria Streptococcus mutans. NaC1, Na_2CO_3, MgSO_4, KC1, MgC1_2, CH_3COOK were examined as anodization solution. When using Na_2CO_3, MgSO_4, and CH_3COOK, anti-bacterial effect was not observed, in contrast, NaC1, KC1, and MgC1_2, were figured out that halogen such as Cl was absolutely imperative for the anti-bacterial effect. Anti-bacterial examination against Streptococcus mutans, Streptococcus gordonii, Streptococcus sobrinus, Escherichi Staphylococcus aureus, Streptococcus pyogenes, Candida albicans, and Candida glabrata showed that it is very effective against bacteria both gram positive and gram negative, however, the effect against fungus such as C, albicans, were less effective. No reduction of the anti-bacterial effect of the anodized titanium plate was observed after immersion in sterile water for 2 weeks, however, after immersion in phosphate buffer saline(PBS) or RPMI1640 medium, the anti-bacterial effect was remarkably reduced. These results suggest that the anti-bacterial effect is based on pH. It might be reasonable that something acid containing Cl, such as HClO was released from the surface of anodized titanium plate. These results indicate that this novel titanium plate anodized by NaCl is effective to prevent the attachment of bacteria and effective to attach the host cells such as osteoblast. This disposition might be very effective for implant use as in this case, we do not have to reuse this material. It is possible that the chance of infection by oral and/or general bacteria is effectively reduced with the implant anodized by NaCl.