Studies on the Structure and Function of Phytochromes as Photoreceptive Chromoproteins Based on Synthetic Organic Chemistry

为了研究光敏色素作为调节植物光形态建成的感光色素蛋白的结构和功能，我们发展了一种高效而灵活的方法来制备光敏色素的发色团(PΦB、PCb和BV)，并对光敏色素有了许多深入的了解。例如，通过化学合成的锁定生色团与附着色素的组装实验，我们可以直接揭示植色农杆菌Agp1和Agp2的红光吸收Pr形式和远红光吸收Pfr形式发色团的立体化学。

Toward investigation of the structure and function of phytochromes as photoreceptive chromoproteins regulating photomorphogenesis in plants, we have developed an efficient and flexible method for the preparation of chromophores (PΦB, PCB, and BV) of phytochromes, and have found a lot of insights into phytochromes. For example, we could directly reveal the stereochemistry of the chromophore in red light absorbing Pr-form and far-red light absorbing Pfr-form of Agrobacterium phytochromes, Agp1 and Agp2, by the assembly experiments of the chemically synthesized locked chromophores with apophytochromes.