Analysis of cis-element and trans- factors relating with responsiveness and metamorphosis of insect

我们分离了角质层蛋白基因BMWCP 2和BMWCP 5的上游区域，并阐明了蜕皮激素反应序列。建立了基因枪法将DNA导入翅盘和翅组织的方法。我们阐明，BR-C Z2，这是一个早期蜕皮激素反应基因，结合并激活启动子活性的BMWCP 10。我们发现EcRE上游的BMWCP 10和EcR的结合EcRE的凝胶电泳迁移率筛选试验和报告分析。我们发现不同的转录因子在表皮的不同区域表达。

We isolated upstream region of cuticle protein genes BMWCP2 and BMWCP5 and clarified the ecdysone responsive sequence. We constructed the method of the introduction of DNA into wing discs and wing tissues by a gene gun. We clarified that BR-C Z2, which is an early ecdysone responsive gene, bound and activated the promoter activity the BMWCP10. We found EcRE upstream of BMWCP10 and showed the binding of EcR to EcRE by a gel electrophoresis mobility sift assay and a reporter assay. We found that the different transcription factors were expressed in different region of the epidermis.