Impairment of memory and neurotoxic mechanism in male mice induced by exposure of domoic acid.

为了解软骨藻酸(DA，健忘性贝毒)的神经毒性机制，我们用雄性小鼠腹腔注射DA。通过恐惧条件反射实验，我们发现了亚急性语境记忆损害和延迟性线索记忆丧失。虽然我们从未检测到组织病理损伤，如神经死亡、神经原纤维缠结、神经丢失，但通过免疫组织化学研究，我们在小鼠的海马区发现了对突触的影响。此外，我们还通过分子生物学方法对小鼠海马区轴突引导信号通路和突触功能的影响进行了研究。

To know the neurotoxic mechanism induced by domoic acid (DA ; amnesic shellfish poison), we administered DA to male mice by intraperitoneal injection. We found subacute impairment of contextual memory and delayed cued memory loss by using fear conditioning test. Although we never detect histopathological damages, i.e. neural death, neurofibrillary tangles, neural loss, we found the effects on synapse by immunohistochemical study in hippocampus of mice. Furthermore, we found the influences on axonal guidance signal pathway and synaptic function in hippocampus of mice by molecular analysis using percellome methods.