Development of forensic neuropathology through a study on brain changes after intracerebral implantation of foreign body

在开放性头部损伤的情况下，异物可能会在创伤后的一段时间内留在大脑中并导致大脑功能障碍。我们开发了一个大脑中有异物的动物模型。将铅或玻璃球植入大鼠的大脑皮层。在植入后12小时至4周的不同时间对大脑进行分析。脑内金属结合蛋白金属硫蛋白（metallothionein）的免疫反应性随着铅球植入时间的延长而显著增加。2周时发现凋亡细胞，4周时凋亡细胞基本消失。此外，在铅球植入后1 ~ 3周，n -甲基- d -天冬氨酸受体基因被显著抑制。本研究表明，铅球浸出铅可诱导脑内金属硫蛋白表达、细胞凋亡和n -甲基- d -天冬氨酸受体基因抑制。

In cases of open head injuries, a foreign body may remain in the brain for a period of time after the trauma and causes brain dysfunction. We developed an animal model incorporating a foreign body in the brain. A lead or a glass ball was implanted in the cerebral cortex of rats. Brains were analyzed at various times between 12 hours and 4 weeks after implantation. The immunoreactivity of metallothionein, a metal binding protein, increased significantly in the brain with increasing time after implantation of a lead ball. Apoptotic cells were identified at 2 weeks, but had mostly disappeared at 4 weeks after implantation of a lead ball. Moreover, N-methyl-D-aspartate receptor genes were significantly suppressed at 1 to 3 weeks after implantation of a lead ball. This study showed that lead leached from a lead ball induces metallothionein expression, apoptosis and N-methyl-D-aspartate receptor gene suppression in the brain.