Development of islet isolation technique to minimize tissue injury by applying nanotechnology

在这项研究中，我们试图开发胰岛分离程序，通过应用纳米技术，特别是胰岛纯化和胰腺分离步骤，以提高分离胰岛的质量和数量。在胰岛纯化步骤中，我们通过流体动力学分析阐明了机械力、剪切力对胰岛破坏的贡献，并通过使用粘度低于标准溶液的纯化溶液成功地提高了胰岛产量。在胰腺分离阶段，我们成功地测量了胰腺在这一步骤中的氧消耗。

In this study, we attempted to develop islet isolation procedure to improve the quality and quantity of isolated islets by applying nanotechnology especially to islet purification and pancreas dissociation steps. On the islet purification step, we clarified by fluid dynamic analysis the mechanical force, shear, contributes to islet destruction and succeed to improve islet yield by using purification solution with lower viscosity than the standard solution. On the pancreas dissociation step, we succeeded to measure O_2 consumption of pancreas during this step.