Molecule targeted therapy for Cholangiocarcinoma based on the gene profiling of cancer stem cell

肿瘤干细胞具有分化和自我繁殖的能力，在肿瘤的发生和发展中起着重要作用，我们利用DNA芯片技术对CD 133阳性和CD 133阴性的HuCCT 1进行了基因表达谱分析。结果未发现CD 133与肝干细胞标志物CD 45、TER、CD 49 f、c-Met之间的关系。该模型的致癌性有显著性差异。我们认为这是多种因素造成的，如癌症起源，另一种癌症干细胞标志物，除了CD 133。此外，我们通过门静脉给药Nek 2 siRNA在裸鼠肝转移中使用的接入端口。我们认识到肝转移的数量减少和肿瘤体积缩小，我们阐明了siRNA通过门静脉的药物递送系统是有效的。

Cancer stem cell, have the ability of differentiation and self-reproduction, is important in carcinogenesis and cancer progression.We analyzed a gene profiling between CD133 positive HuCCT1 and the CD133 negative HuCCT1 by DNA array. As a result, we were not able to find the relationship between CD133 and hepatic stem cell marker, such as CD45, TER, CD49f, and c-Met.And then, we inoculated CD133 positive HuCCT1 and the CD133 negative HuCCT1 in the nude mouse. There was significant difference of carcinogenesis in this model. We considered that this caused in various factors, such as the cancer origin, another cancer stem cell marker except CD133.In addition, we administered Nek2 siRNA in liver metastasis of a nude rat through portal vein using the access port. We recognized decrease of the number of the liver metastasis and the reduction of the tumor volume.We clarified that the drug delivery system of siRNA through the portal vein was effective.