Molecular mechanism and physiological function of glutathione-dependent regulation of plastidic aldolase

谷氨酸调节植物生理事件的各个方面。为了了解谷胱甘肽依赖的生理事件的机制，我们集中在质体醛缩酶，蛋白质进行谷胱甘肽化。拟南芥基因组中有3个编码质体果糖-1，6-二磷酸醛缩酶的基因。谷胱甘肽结合同工酶FBA 1在数量上是次要的（占叶片中质体FBA蛋白总量的2%），并被归类为一个与其他两种同工酶FBA 2和FBA 3不同的遗传分支，这两种同工酶FBA 2和FBA 3通常被认为参与了卡尔文循环。然而，只有FBA 1活性被谷胱甘肽上调，FBA 1的减少增加了对光的敏感性。总之，卡尔文循环涉及新的同工酶。

Glutathione regulates various aspects of physiological events in plants. In order to understand the mechanism underlying the glutathione-dependent physiological events, we focused on a plastidic aldolase, one of proteins undergoing glutathionylation. There were three genes encoding plastidic fructose-1,6-bisphosphate aldolase in the Arabidopsis genome. The glutathione-binding isozyme FBA1 was quantitatively minor (2% of the total plastid FBA proteins in the leaf) and categorized into a phylogenic branch different from that containing the other two isozymes FBA2 and FBA3, which were conventionally considered to be involved in the Calvin cycle. However, only FBA1 activity was upregulated by glutathione and the decrease in FBA1 increased the sensitivity against light. Taken together, the Calvin cycle involves new isozyme.