Discrimination method for Takifugu hybrids using AFLP and mtDNA analyses

通过mtDNA全序列分析，阐明了硬骨鱼中四齿形目鱼类、四齿形目鱼类和河豚属鱼类的系统发育关系。利用AFLP技术对9种河豚进行了杂交分析。根据物种特异的AFLP标记，除红毛委陵菜和中国毛委陵菜之间的物种外，其他所有物种都能被明显区分。他们的杂交种也被区分开来，47个杂交种中有46个是F1代的杂交种。为了鉴定河豚杂交种和物种，需要开发简便的特异AFLP标记的引物。

Phylogenetic relationships of tetraodontiform fishes within teleosts, of tetraodontiform fishes, and of Takifugu species were clarified using mtDNA full sequences analysis. Hybrid analysis was also performed among 9 Takifugu species using AFLP method. All species other than between T.rubripes and T.chinensis were clearly distinguishable based on species specific AFLP markers. Their hybrids were also discriminated and 46 out of 47 hybrids were those of F1 generation. Developing of convenient primers for specific AFLP markers is needed in order to identify Takifugu hybrids as well as species.