Transkriptional regulators of jasmonate-induced alkaloid biosynthetic genes in Eschscholzia californica

生物碱是一类生理活性次生代谢物，可在植物和植物细胞培养物中积累，以响应伤害和/或激发子处理。在多种植物中，苯并[c]菲生物碱生物合成途径的激活是通过脂膜衍生的十八烷酸(12-氧代植物二烯酸/茉莉酸)信号转导途径的转录激活进行的。在马达加斯加长春花长春中，最近发现了茉莉酸甲酯诱导的AP2结构域转录因子(Orcas)，它们能够调控单萜类吲哚生物碱的生物合成。这项建议的目的是更多地了解这种类型的转录因子在苯并[c]菲生物碱生物合成基因激活中的作用。我们打算研究的系统是茉莉酸甲酯诱导加州罂粟细胞悬浮培养中生物碱生物合成基因激活的系统。

Alkaloids are a class of physiologically active secondary metabolites that can be accumulated in plants and plant cell cultures in response to wounding and/or elicitor treatment. The activation of the benzo[c]phenanthridine alkaloid biosynthetic pathway in a variety of plant species proceeds by transcriptional activation via signal transducers that are lipid membranederived octadecanoids (12-oxo-phytodienoic acid / jasmonic acid). In the Madagascar periwinkle Catharanthus roseus, methyl jasmonate-inducible AP2-domain transcription factors (ORCAs) have recently been identified that demonstrate the ability to regulate genes of monoterpenoid indole alkaloid biosynthesis. It is a goal of this proposal to learn more about the role of this type of transcription factor in benzo[c]phenanthridine alkaloid biosynthesis gene activation. The system that we propose to investigate is that of methyl jasmonate-induced alkaloid biosynthetic gene activation in cell suspension cultures of the California poppy Eschscholzia californica.