Development of the molecular imaging for proteins related to neuropathic pain

我们对小鼠囊性GABA转运蛋白异质性（VGAT+/-）进行分析，探讨GABA和甘氨酸的变化规律。小鼠似乎从突触前部位释放较少的GABA和甘氨酸。我们研究了WT小鼠和VGAT+/-小鼠的麻醉敏感性和感觉表现，比较了急性伤害感受和炎症性疼痛试验。此外，我们还通过电生理特性检测了gaba能和甘氨酸能微型抑制性突触后电流（mIPSC）。比较野生型（WT）小鼠和VGAT+/-小鼠的麻醉作用时间和麻醉作用持续时间。为了测试棘上水平的感觉表现，我们测量了从52℃的热板中跳跃或舔的潜伏期。为了检测炎症性疼痛信号，我们使用了福尔马林试验。现在我们研究gaba能和甘氨酸能的mIPSC，并比较两种基因型在两种电流中的衰减和频率。

We analyzed vesicular GABA transporter heterogenic(VGAT+/-) mice to find out the rule of GABA and glycine. The mice are seemed to have less release of GABA and glycine from presynaptic sites. We studied anesthetic sensitiveness and to examine sensory performance, acute nociception and inflammatory pain tests were compared between WT mice and VGAT+/- mice. Also we examined GABAergic and glycinergic miniature inhibitory postsynaptic currents (mIPSC) by electrophysiological properties. The time to anesthetic effect nor the duration of anesthetic effect were compared between wild-type (WT) mice and VGAT+/- mice. To test the sensory performance at the supraspinal level, we measured the latency to jumping or licking from the hotplate set at 52℃. To examine about inflammatory pain signaling, we used the formalin test. Now we examine GABAergic and glycinergic mIPSC and compare abut decay and frequencies in two currents of both genotypes.