The effect of fasudil, a Rho-kinase inhibitor, on cerebral microcirculation under total transient cerebral ischemia in rabbits.

本实验研究了Rho-Kinase抑制剂法舒地尔对兔短暂性全脑缺血后脑微循环的影响。用颈止血带在日本大白兔的颞骨上开颅窗，造成短暂性全脑缺血。我们观察了动脉二氧化碳分压升高和异氟醚(一种挥发性麻醉剂)浓度升高引起的大脑软脑膜动脉的生理变化。法舒地尔对脑缺血前后的反应程度均无影响，未观察到CO2和异氟醚对脑缺血后血管的扩张作用。此外，法舒地尔不改变大脑小动脉在亚低温下对二氧化碳和异氟醚的反应。结论：法舒地尔本身对脑微循环生理性改变无明显影响。

We studied the effect of fasudil, a Rho-kinase inhibitor, on cerebral microcirculation under total transient cerebral ischemia in rabbits. Cranial window was made on a temporal bone of Japanese White Rabbits with neck tourniquet to induce transient global cerebral ischemia. We observed cerebral pial arteioles' physiological changes induced by increased arterial carbon dioxide tension and the concentration of isoflurane, a volatile anesthetic. Fasudil did not change the degree of response either before or after cerebral ischemia, which we failed to observe cerebral arteriovasodilation by CO2 and isoflurane after ischemia. Furthermore, fasudil did not alter the response of cerebaral arterioles to CO2 and isoflurane under mild hypothermia. We concluded that fasudil itself did not impact on physiologic changes of cerebral microcirculation.