Distribution and importance of extracellular DNA in the subsurface sediments

胞外DNA定量与陆地地下沉积物。检测到的细胞外DNA含量为2.9-28.3纳克/克沉积物干重，并随深度增加而减少。使用胞外DNA的微生物群落分析产生显著低数量的OTU，表明胞外DNA在环境中被片段化或损坏。基于RNA的群落分析显示，在陆地地下环境中的活性微生物（变形菌，绿弯曲菌，酸杆菌，杂项Crenarchaeotic组（MCG），ANME）的多样性。

Extracellular DNA was quantified with terrestrial subsurface sediments. Extracellular DNA was detected at 2.9-28.3 ng/g dry weight sediment and decreased with depth. Microbial community analysis using the extracellular DNA yielded significantly low number of OTUs, suggesting that the extracellular DNA was fragmented or damaged in the environment. RNA based community analysis revealed variety of active microbes (Proteobacteria, Chloroflexi, Acidobacteria, Miscellaneous Crenarchaeotic Group (MCG), ANME) in the terrestrial subsurface environment.