Characteristics of Oral Tissue-Derived MSCs from CLP Patients

尽管越来越多的报道将MSCs用于CLP患者的牙槽腭骨重建，但目前尚不清楚CLP患者的口腔组织来源的MSCs是否能够分化为成骨细胞并再生出与其匹配的对照组相当的新骨。本研究旨在探讨唇腭裂患者牙周膜干细胞的特点。更具体地说，该研究分析了来自CLP患者的这些细胞的自我更新活性和多系分化潜力，并与健康对照组进行了比较。对16例患者的24颗拔牙进行了分析。11颗牙齿(CLP患者)和13颗牙齿(对照健康患者)。这些牙齿在正畸治疗前被指定拔牙，但因恒牙状况而拔除的乳牙除外。CLP患者DPCS和PDL细胞的细胞增殖与对照组无差异。而CLP组DP细胞与PDL细胞比较，第1天PDL细胞增殖明显高于DP细胞(P&lt；0.05)。CLP组细胞的PDT与正常对照组相当。CFU检测也有类似的观察结果。茜素红染色、碱性磷酸酶(ALP)、Runx2和骨桥蛋白(OPN)的表达检测DPSCs向成骨细胞方向分化的能力。在成骨细胞培养液中，CLP组和对照组细胞内均有钙沉积。在基础培养液中培养的细胞未见染色。以MC3T3-E1成骨样细胞为阳性对照。

Despite increasing reports on the use of MSCs for alveolar palate bone reconstruction in CLP patients, it remain unclear whether MSCs of oral tissue origin taken from CLP patients are capable to differentiate into osteoblastic lineage and regenerate new bone comparable as to its matched controls. This study aims to examine the characteristics of DPSCs taken from cleft lip palate patients. More specifically, the study analyze the self-renewal activity and multi-lineage differentiation potential of these cells from CLP patients in comparison to healthy matched controls. Analyses were carried out from 24 extracted tooth were collected from 16 patients. 11 teeth (CLP patients) and 13 teeth (control healthy patients). The teeth were indicated for extraction prior to orthodontic treatment, except for deciduous teeth that were extracted due to persistence condition. No differences in the cell proliferation of DPCSs and PDL Cells between CLP patients and control. However comparison of DP cells and PDL cells in CLP group showed higher cells proliferation at Day 1 in PDL cells compared to DP Cells (P<0.05). PDT of cells from CLP group were comparable with control healthy group. Similar observation was seen for CFU assay. The ability of DPSCs to differente into osteoblastic lineage was examined by Alizarin red staining, ALP, Runx2 and OPN mRNA expression. Calcium deposition were detected in cells from CLP and Control group cultured in osteogenic medium. No staining was seen in the cells cultured in basal medium. MC3T3-E1 osteoblast-like cells were used as positive control.