Identification of neurons by fluorescence spectrogram through a patch electrode, and the application to auditory neural circuits in vivo

本研究的目的是在脑深部组织中同时记录光信号和神经元电活动。为此，我们开发了一个光度贴片电极（PME）记录系统，通过使用贴片电极作为光导。用俄勒冈州绿色BAPTA-1-AM（OGB-1）填充鸡听觉核团的神经元，在Field-L、鸟类听觉皮层和下丘中同时记录细胞内Ca 2+信号和响应于声刺激的电信号。在Field-L中阻断GABA受体后，慢场电流增强，这增强了与电慢波同步产生的Ca 2+波。在下丘，同样记录到Ca ~（2+）反应，并在GABA受体阻断后增强。因此，我们成功地开发了PME记录系统，通过贴片电极在脑深部组织中同时监测神经元电活动和光信号。

This study aimed at recording optical signal simultaneously with neuronal electrical activity in the deep brain tissue. For that purpose, we developed a photometric patch electrode (PME) recording system by using a patch electrode as a light guide. By filling the neuron in the auditory nuclei of a chick with Oregon green BAPTA-1-AM (OGB-1), intracellular Ca2+signals were recorded simultaneously with electrical signal in response to acoustic stimulus in the Field-L, the avian auditory cortex, and in the inferior colliculus. A slow field current was enhanced after the block of GABA receptor in the Field-L, which enhanced the Ca2+wave that was generated in synchrony with the electrical slow wave. In the inferior colliculus, Ca2+responses were similarly recorded and were enhanced after the block of GABA receptor. Therefore, we succeeded in development of PME recording system to monitor neuronal electrical activity simultaneously with optical signal through the patch electrode in the deep brain tissue.