Identification of novel DOPA ligands and electrophysiological analysis of DOPA-induced response

多巴是中枢神经系统中一种新的神经递质候选者。为了确定多巴的特异性受体(S)，我们寻找了选择性和稳定的多巴配体。在麻醉大鼠孤束核(NTS)内微量注射L-苏二羟基苯丝氨酸溶液，发现一种能引起降压和心动过缓反应的阳性部位。在多电极阵列系统中，我们还在大鼠脑干脑片NTS区发现了对DOPA的双相反应。在进行这一项目时，我们新确定了DOPA的候选受体。

DOPA is a novel neurotransmitter candidate in the central nervous system. In an attempt to identify a specific receptor(s) for DOPA, we have searched for selective and stable DOPA ligands. We found a positive fraction in L-threo-dihydroxyphenyserine-containing solution to induce depressor and bradycardic response when microinjected into the nucleus tractus solitarii (NTS) of anesthetized rats. In multiple electrode array system, we also found biphasic response to DOPA in NTS area of rat brain stem slices. While conducting this project, we newly identified a receptor candidate for DOPA.