Development of high sensitive and high S/N ratio multicolored SNP detection technology toward each locus.

智能扩增(SmartAmp、SMAP)基因分型系统的双色读数已被开发出来，该系统使用所谓的激子引物。新方法具有高度的特异性和敏感性，允许一步、单管突变检测反应，直接从血液中得出结果只需45分钟。作为“序列特异性荧光发射”的激子引物，在与互补序列杂交后，可以为扩增反应的实时监测提供目标序列检测的信号。在等温SmartAmp2突变检测过程中，激子引物表现出高信号强度和低背景，与SYBR Green I相比具有更高的特异性和灵敏度。基因分型分析可以只使用一个标记的激子引物进行终点检测，或同时使用两个具有不同染料的激子引物在一管反应中同时检测野生型和突变型等位基因。由于该方法不涉及复杂的仪器和样品提纯，因此该技术对于分散的医疗保健点诊断似乎非常有前景。我们以SNPs和突变的快速检测为例，展示了新技术的有效性

A two-color readout of the Smart Amplification (SmartAmp、SMAP) genotyping system using so-called Exciton Primers have been developed. New method is highly specific and sensitive, permitting a one-step, single-tube mutation detection reaction which delivers a result in 45 minutes directly from blood. Exciton Primers, which are functioning as "fluorescence emission with sequence-specific," after hybridization to complementary sequences, can provide the signals resulting target sequence detection for real-time monitoring of amplification reactions. Applied to the isothermal SmartAmp2 mutation detection process, Exciton Primers show high signal strength with low background leading to a superior specificity and sensitivity compared to SYBR Green I. The genotyping assay can use only one labeled Exciton Primer for endpoint detection, or simultaneously by real-time monitoring detect wild-type and mutant alleles in a one-tube reaction using two Exciton Primers having different dyes. The technique seems very promising for decentralized point-of-care diagnostics since the approach does not involve complicated instrumentation and sample purification. We demonstrated efficiency of the new technology on the example of rapid detection of SNPs and mutations