Regulation of endosome formation and recycling by Cdk5/p35 in neurons

CIN85是参与内吞作用的衔接蛋白，AATYK1是在神经元中高度表达的Ser/Thr激酶。我们最近发现，这两个都被Cdk5磷酸化。我们研究了Cdk5磷酸化在神经元中的作用。AATYK1被证明调节再循环内体运输和活性被Cdk5磷酸化抑制。AATYK1活性参与轴突伸长。CIN85的功能尚不清楚。我们产生了一种特异性抗体来研究内源性CIN85在神经元中的定位。CIN 85以小颗粒形式存在于神经元索马和突起中，被认为是非常早期的内吞结构。

CIN85 is an adapter protein involved in endocytosis and AATYK1 is a Ser/Thr kinase expressed highly in neurons. We found recently that both were phosphorylated by Cdk5. We studied roles of their Cdk5 phosphorylation in neurons. AATYK1 was shown to regulate recycling endosomal trafficking and the activity was suppressed by phosphorylation with Cdk5. AATYK1 activity is involved in axonal elongation. The function of CIN85 is not known. We generated a specific antibody to investigate endogenous CIN85 localization in neurons. CIN85 was present as small particles in neuronal soma and process, which were thought to be very early endocytic structures.