Molecular analysis of hormonal orchestration that induces various seasonal polyphenisms

为了分析淫羊藿（Orgyia the llina）生殖多表型（包括滞育诱导）的分子机制，我们首次克隆并鉴定了编码5个phex - pro - arg - leu - nh_2 （FXPRLa）神经肽的滞育激素-信息素生物合成激活神经肽（DH-PBAN） cDNA。我们发现，不仅Orgyia DH能诱导胚胎滞育，而且这种神经肽还能诱导季节性多表型，参与卵泡和卵巢的肥大。这是首次研究表明神经肽在季节性生殖多表型中具有多效性作用，以完成季节适应。我们还证明了一个新的因素(i。例如，DH神经肽)是季节性多表型的重要诱导剂，是生活史权衡的基础。

To analyze the molecular mechanisms involved in reproductive polyphenism, including the diapause induction of Orgyia the llina, we first cloned and characterized the diapause hormone-pheromone biosynthesis activating neuropeptide(DH-PBAN) cDNA encoding the five Phe-X-Pro-Arg-Leu-NH_2(FXPRLa) neuropeptides. We revealed that not only does the Orgyia DH induce embryonic diapause, but also that this neuropeptide induces seasonal polyphenism, participating in the hypertrophy of follicles and ovaries. This is the first study showing that a neuropeptide has a pleiotropic effect in seasonal reproductive polyphenism to accomplish seasonal adaptation. We also show that a novel factor(i. e., the DH neuropeptide) acts as an important inducer of seasonal polyphenism underlying a life-history tradeoff.