The development of new control technology in CO2 and N2O emission by symbiotic microorganisms.

我们选择了一种即使在胁迫条件下也能促进寄主植物生长的暗隔内生真菌(DSE)的关键种--Fortinii Phialocephala fortinii。我们认为，需要在苗圃中建立适当的生长条件，包括适当的营养和碳浓度，以确保DSE真菌与寄主植物之间形成共生关系。通过添加氨基酸，进一步提高了Fortinii的定殖率。筛选出了属于DSE的两株单纯韦氏杆菌Y34和IBAK45。有趣的是，荧光显微镜观察到两个菌株的菌丝内外都存在细菌群落，菌丝体内毒素的检测也进一步证实了这一点。我们试图通过持续的抗生素治疗和物理清除的方法来控制这些伴生菌，以阐明这些伴生菌的作用。末端限制性片段长度多态性(T-RFLP)结合16S rDNA基因克隆的序列数据，在两株真菌野生株的变形杆菌中检出了假单胞菌属和单胞菌属，而蓝藻中的微囊藻属和变形杆菌中的Delftia属分别在IBAK45和Y34野生株中检测到。

We selected a key species of the dark septate endophytic fungus (DSE), Phialocephala fortinii, which promotes the growth of host plant even under the stressed conditions. We showed that the appropriate growth conditions in nursery set including suitable nutrient and carbon concentrations need to be developed to ensure the formation of the symbiotic association between the DSE fungi and the host plants. The improvement in the colonization rate by P. fortinii was confirmed by adding the amino acid.Two strains of Veronaeospsis simplex, Y34 and IBA K45, which belong to the DSE have been selected. Interestingly, the presence of bacterial communities living outside and inside mycelium of both strains was revealed by fluorescence microscopic observation and confirmed further by the detection of endotoxin in mycelium as well. We attempted to control these associated bacteria using continuous antibiotic treatment and also using physical removal method to clarify the role of these associated bacteria. Terminal-restriction fragment length polymorphism (T-RFLP) coupled with the sequence data from 16S rDNA gene cloning detected the genera Pseudomonas and Stenotrophomonas in the Proteobacteria on both fungal wild strains, while the genus Microcystis in the Cyanobacteria and Delftia in the Proteobacteria were only detected in wild strain of IBA K45 and Y34, respectively.