Establishment of minimally invasive cell therapy for diabetes by improving the outcome of pancreatic islet transplantation

在目前的研究中，我们已经阐明了在分离过程中脑死亡与热缺血应激相结合可诱导离体胰岛中炎症介质的高表达。我们还发现C5a抑制肽通过抑制受体肝脏中白细胞组织因子表达的上调来减弱补体和凝血级联之间的串音，这一过程导致胰岛移植的改善。这是一个改善胰岛移植预后的新概念。我们还表明，异氟醚和短期禁食联合胰岛素治疗可能是一种有希望改善肝脏胰岛移植的方案。

In the present study, we have clarified that brain death in combination with warm ischemic stress during isolation procedures induces a high expression of inflammatory mediators in isolated islets. We also found that C5a inhibitory peptide attenuates cross-talk between the complement and coagulation cascades through suppressing up-regulation of tissue factor expression on leukocytes in recipient livers, a process leading to improvement in islet engraftment. This was a new concept to improve the outcome of islet transplantation. We also showed that isoflurane and short-term fasting combined with insulin treatment could be a promising regimen for improving islet engraftment in the liver.