Synthesis of Cello-Oligosaccharides for Alcohol Fermentation by Hydrolysis of Crystalline-Region Cellulose

找到了一种实用的水解结晶纤维素的方法。将结晶纤维素水悬浮液在氢键减弱的260℃±10℃下加热后进行汽爆。所得纤维素显示水解仅发生在其表面。丝光纤维素也表现出同样的现象。结晶纤维素在200℃左右发生酸水解，转变成不同的结晶状态，得到单糖和寡糖衍生物，表明发生了主链断裂。转化后的纤维素容易被酶水解成单纤维素和低聚纤维素。

A practical method to hydrolyze crystalline cellulose was found out. After crystalline cellulose suspension in water was heated at 260℃±10℃ in which temperature the hydrogen bond is weakened, it was steam-exploded. The obtained cellulose showed that hydrolysis occurred only at its surface. Mercerized cellulose showed the same phenomenon. For the crystalline cellulose converted into a different crystalline state, an acidic hydrolysis around 200℃ gave mono- and oligo-saccharide derivatives, indicating the occurrence of main-chain scission. The converted cellulose was easily hydrolyzed by enzymes into monoand oligo-saccharides.