Molecular mechanism of genome maintenance in Thermus thermophilus

我制备了参与错配修复和碱基切除修复系统的Thermus thermophilus HB8蛋白，并进行了修复反应的重组、蛋白质-蛋白质相互作用分析和晶体结构分析。基于这些结果，我揭示了错配修复蛋白之间的新的相互作用有助于链识别，降解错误链的核酸酶通过其独特的结构识别单链DNA，以及两种DNA聚合酶在不同的碱基切除修复途径中发挥作用。

I prepared Thermus thermophilus HB8 proteins involved in mismatch repair and base excision repair systems and performed reconstitution of repair reactions, protein-protein interaction analysis and crystal structural analyss. Based on the results, I revealed that novel interactions between mismatch repair proteins contribute to strand discrimination, the nuclease which degrades an error-strand recognizes single-straded DNA by its unique structure, and two DNA polymerases function in different pathways of base excision repair.