Development of the safe and functional avirulent neurotracing virus producing system.

测定了嗜神经性冠状病毒猪血凝性脑脊髓炎病毒（PHEV）67N株的全基因组序列，为反向遗传学研究PHEV神经示踪病毒奠定了基础。嗜神经性67N株ns2基因有400个碱基插入，非嗜神经性OSN 204株ns2基因有200个碱基缺失。体外感染神经母细胞瘤和胶质瘤细胞的结果表明，PHEV 67 N株具有高度嗜神经性。

The entire genome sequence of the porcine hamagglutinating encephalomyelitis virus (PHEV) 67N strain, neurotropic coronavirus, was determined, and the sequence data is useful for reverse genetics of PHEV to develop the PHEV based neurotracing virus. There is about 400 base insertion in ns2 gene of neurotropic 67N strain and 200 base deletion in ns2 gene of non neurotropic OSN204 strain. The results of in vitro PHEV infection to Neuroblastoma and glioma cell line indicated that PHEV 67N strain have high neurotropism.