Analysis of the Influence and Measure of Chytrid fungus (Batrachochytrium dendrobatidis) for native amphibian species in Japan

我们在亚洲首次发现壶菌病。通过大规模死亡病理检查和本地两栖动物感染实验，明确了日本本地野生两栖动物中壶菌（Batrachochytrium dendroatidis: Bd）的情况和本地物种对壶菌的抗性。我们还通过患病动物和职业实验建立了有效和安全的治疗/根除Bd的方法。从6例两栖动物大规模死亡中分离并建立了2株ranv病毒（RCV-JP和HNV）。通过11种本地种的病毒攻毒实验，证实了高致死率（84.0%）。

We discovered chytridiomycosis for the first time in Asia. The situation of chytrid fungus (Batrachochytrium dendrobatidis : Bd) in native wild amphibians in Japan and the resistance of native species to Bd were clarified by the pathological examination of mass die-offs and the infection experiment using native amphibians. We also established effective and safe treatment/eradication of Bd by experiments using affected animals and career. Two ranavirus strains (RCV-JP and HNV) were isolated and established from 6 mass die-offs of amphibian. And, high mortality rate (84.0%) was clarified by the ranavirus challenge experiments using 11 native species.