Saccharification of biomass with enzymes fused to carbohydrate-binding modules

纤维素结合模块，家庭3，17，28，和44，其特征在于与宏观阵列方法，等温滴定量热法;和Langmuir等温分析。为了确定植物细胞壁上的结合位点，将这些纤维素结合模块与荧光蛋白融合。荧光融合纤维素结合模块可与植物细胞壁不同部位结合，区分植物组织的细胞类型。这些结果表明，纤维素结合模块可以识别植物细胞壁上的不同结构，而这些模块具有相似的结合特异性。属于糖苷水解酶家族5的催化结构域融合到纤维素结合模块。这些人工酶的活性均受到融合模块的影响。

Cellulose-binding modules, family 3,17,28, and 44, were characterized with the macro-array method, isothermal titration calorimetry;and Langmuir isotherman alysis. To determine binding-sites on plant cell wall, these cellulose-binding modules were fused to a fluorescent protein. Fluorescent fused cellulose binding modules bound to different parts of plant cell wall and discriminated cell types of plant tissues. These results indicated that cellulose-binding modules can recognize a diffbrent structure on plant cell walls, whereas these modules have similar binding specificities. A catalytic domain belonging to glycoside hydrolase family 5 was fused to cellulose binding modules. These artificial enzymes showed their activities were influenced by fused modules.