Distinct way to prepare the hyper-stable and stress-tolerant enzymes-halophilic enzyme engineering and hyper-functional cell production

嗜盐酶的氨基酸组成通常以酸性氨基酸的丰富含量为特征，导致高水溶性，而在天然和变性状态下均不聚集，因此允许嗜盐蛋白在热或变性剂处理后有效复性。用酸性氨基酸残基取代非嗜盐蛋白中的中性或碱性氨基酸残基，将非嗜盐蛋白转化为嗜盐蛋白，其显示出更高的水溶性和稳定性。

The amino acid composition of halophilic enzymes is in general characterized by an abundant content of acidic amino acid leading to high aqueous solubility without aggregation in both native and denatured states and hence allowing efficient renaturation of halophilic proteins after heat or denaturant treatment. The replacement of neutral or basic amino acid residues in non-halophilic proteins with acidic amino acid residues converted non-halophilic to halophilic proteins which show higher aqueous solubility and stability.