Study on the kinetics of soluble fibrin in blood and the utility of SF measurement in thrombosis.

血栓形成患者血液中存在多种可溶性纤维蛋白(SF)复合体。我们研究了不同的SF复合体中哪些在血栓形成前立即增加，以及该复合体是否是血栓形成的预测因素。血栓形成倾向和急性心肌梗死患者在血栓形成早期血浆FMC显著升高。我们的结果表明，小血管内血栓的形成可以在FMC水平上反映出来。经0.20μm和0.45μm滤膜处理的样品中SF的测定表明，在早期样品中SF络合物的大~中分子增加。用抗纤维蛋白单体的单抗F405进行Western印迹分析时，经等电聚焦电泳后，在大范围内(PH5~10区域)检测到不同相对分子质量的SF，提示血栓形成早期SF复合体的血流动力学发生了变化。

Various complexes of soluble fibrin (SF) are present in blood of patients with thrombosis. We investigated which in various complexes of SF increase immediately prior to the formation of thrombus, and whether the complex is a predictive factor for thrombosis. In patients with thrombotic tendency and with acute myocardial infarction, plasma FMC was significantly elevated in the early stage of thrombosis. Our results suggested that the thrombus formation in a small blood vessel can be reflected on FMC level. The determination of SF in the sample treated with 0.20μm or 0.45μm filter showed that the large～intermediate molecule of SF complexes increased in the early stage samples. When western blot analysis using monoclonal anti-fibrin monomer antibody, F405, after the isoelectric focusing electrophoresis, SF of various molecular weight were detected within the wide range (pH5～10 area) in early stage samples, suggesting the variety in blood kinetic of SF complexes in early stage of thrombus formation.