Development of diagnostic and therapeutic strategies for gastrointestinalcancer : focus on tumor-associated ribosomal proteins

我们在这里报道L13在人类胃肠道癌症中被激活。通过qRT-PCR，我们分析了L13在刚切除的胃、结直肠和肝脏癌组织中的表达。与邻近正常组织相比，36例胃癌组织中有10例（28%）、46例结直肠癌组织中有19例（41%）、25例肝癌组织中有5例（20%）的L13 mRNA表达上调。我们还发现L13基因的表达增加与胃癌的临床分期相关。重要的是，我们发现原发性胃癌中L13的表达与p53突变状态呈负相关，sirna介导的L13抑制导致p53蛋白的强烈诱导。这些发现表明L13通过麻痹p53通路在一些胃肠道恶性肿瘤的进展中起重要作用，从而在野生型p53存在下允许致瘤性生长。

We report here that L13 is activated in human gastrointestinal cancers. By using qRT-PCR, we analyzed expression of L13 in freshly resected cancer tissue of the stomach, colorectum, and liver. Upregulation of L13 mRNA expression was observed in 10 (28%) of 36 stomach, 19 (41%) of 46 colorectal, and 5 (20%) of 25 liver cancer tissue samples compared to adjacent normal tissue samples. We also found that increased expression of the L13 gene was correlated with clinical staging in stomach cancers. Importantly, we discovered that L13 expression was inversely related to p53 mutational status in primary gastric cancer, and siRNA-mediated L13 suppression resulted in robust induction of p53 protein. These findings suggest that L13 plays an essential role in the progression of some gastrointestinal malignancies by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53.