An evolution of mammalian viviparity by retrotransposon

我以前已经确定Peg10作为一种新的印记基因。此外，我已经证实，Peg10是一个逆转录转座子衍生的基因，对胎盘发育具有重要的功能。在本研究中，为了揭示Peg10在胎盘中的作用机制，我从Peg10 KO小鼠中建立了Peg10 KO TS（滋养层干细胞）细胞系。通过比较Peg10 KO TS细胞系和野生型TS细胞系的基因表达，成功分离了Peg10下调基因。这些基因对哺乳动物胎生的进化一定很重要。

I have previously identified Peg10 as a novel imprinted gene. Moreover, I have confirmed that Peg10 is a retrotransposon-derived gene and have an essential function for placental development. In this grant, to reveal the mechanism of Peg10 function in placenta, I have established Peg10 KO TS (Trophoblast Stem) cell lines from Peg10 KO mice. By comparing the gene expression between Peg10 KO TS cell lines and wild type TS cell lines, I could succeed in the isolation of Peg10 down regulated genes. These genes must be important for the evolution of mammalian viviparity.