Study of regulatory mechanism of intracellular vesicle transport by motor proteins using 3-dimensional tracking of nano-particle.
Study of regulatory mechanism of intracellular vesicle transport by motor proteins using 3-dimensional tracking of nano-particle.
利用纳米颗粒的 3 维跟踪研究运动蛋白对细胞内囊泡运输的调节机制。
批准号：
20770116
负责人：
TAKETOSHI Kambara
金额：
$2.66万
依托单位：
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
项目状态：
已结题
起止时间：
2008 至 2009
中文摘要
翻译
马达蛋白在将细胞器和囊泡运输到目的地的过程中起着重要作用。本课题的目的是构建新的系统来理解基于运动的细胞内转运的调节机制。我纯化了重组马达蛋白，并可以获得附着在这些蛋白上的荧光纳米颗粒。纳米颗粒被诱导成半完整的细胞，我可以成功地以高时空分辨率跟踪这些细胞的三维运动。
英文摘要
Motor proteins play an important role in transporting organelles and vesicles to the destination. The goal of this project is to construct new system to understand the regulatory mechanism of motor-based intracellular transport. I purified recombinant motor proteins, and could obtain fluorescent nano-particles attached to these proteins. The nano-particles were induced to semi-intact cells, and I could successfully track the 3-dimensional movement of these in high speciotemporal resolution.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
海外基金
