Analysis of gene silencing mechanism by bacterial small RNA

SgrS sRNA通过与ptsG mRNA的碱基配对作用，导致ptsG mRNA的翻译抑制和降解。我们发现SgrS和ptsG mRNA之间的碱基配对是SgrS介导的翻译沉默的直接原因。我们还发现了一个最小的SgrS碱基配对区域，这是翻译抑制ptsgmrna所必需的。这些结果有助于理解hfq依赖性sRNA在细菌中作用的分子机制。

SgrS sRNA acts by baseparing with ptsG mRNA, resulting in translation inhibition and degradation of ptsG mRNA. We showed that the base pairing between SgrS and ptsG mRNA is directly responsible for translational silencing mediated by SgrS. We also identified a minimal base-pairing region of SgrS required for translational repression of ptsG mRNA. These results contribute to understanding the molecular mechanism by which Hfq-dependent sRNA act in bacteria.