Identification of regulators controlling cellulase and hemicellulase gene expression in filamentous fungi and its functional analysis.

我们报道了根癌农杆菌介导的棘孢曲霉功能基因组学转化的成功。我们分离了40个转化子，这些转化子预期是控制A.从通过AMT获得的15，000个转化体中分离得到Aculeatus。A基因突变后，纤维二糖水解酶基因在大肠杆菌中的表达量降低了5倍。目前，我们正在进一步分析本研究中发现的转录因子的功能，并寻找其他的调控因子。

We report the successful Agrobacterium tumefaciens-mediated transformation (AMT) of Aspergillus aculeatus for functional genomics. We isolated 40 transformants expected to be disruptants of the regulator(s) controlling cellulase gene expressions in A. aculeatus from 15,000 transformants obtained by AMT. Mutation of the geneA resulted in 5-fold reduction on cellobiohydrolase gene expression in transformant by comparing with that in host strain. Now, we are further analyzing the function of putative transcription factor identified in this study and seeking the other regulators.