The regulatory system of methanol metabolism by oxygen in methylotrophic yeast Pichia methanolica.

甲醇代谢途径是氧气反应系统的一个很好的模型，因为它需要和消耗氧气。甲醇代谢在甲基养酵母、甲醇毕赤酵母中进行，大量的氧气用于甲醇氧化和呼吸链中ATP的合成。本研究探讨了毕赤酵母CYC1的表达模式。PmCYC1的表达水平随甲醇浓度的变化而变化，在高浓度甲醇上生长时，CYC1表达被强烈诱导。这些结果表明，PmCYC1的这种表达模式变化与甲醇代谢活性有关。在真核生物中，染色质重塑系统在转录调控中起着重要作用。为了了解组蛋白乙酰化是否在AOD的调控中起作用，我们测试了组蛋白减速酶（hdac）激活剂的作用。结果表明，白藜芦醇是一种有效的HDAC激活剂，可诱导AOD的表达。

Methanol metabolic pathway is a good model for oxygen response system as it requires and consumes oxygen. Methanol metabolism in methylotrophc yeast, Pichia methanolica a large amount of oxygen are used for methanol oxidation and for synthesis of ATP in respiratory chain. In this study the author investigated that Pichia methanolica CYC1 expression patterns. The level of PmCYC1 expression changed with methanol concentration, and CYC1 expression was induced strongly at high oxygen concentration when grown on methanol. These results showed that, this expression pattern change of PmCYC1 is specific to methanol metabolizing activity. In eukaryotes chromatin remodeling system is important in transcriptional regulation. To find out whether histone acetylation plays a role in the regulation of AOD we tested the effect of histone decelases (HDACs) activator. As a result the expression of AOD induced by Resveratrol which is a potent activator of HDAC.