Construction of D-aminoacyl peptides synthetase

含有d -氨基酸的肽有望增加肽的功能多样性和应用。在这项研究中，我们构建了突变的d -氨基肽酶，作为合成含d -氨基酸肽的便利工具。我们获得了一种丝氨酸d立体特异性的氨基水解酶，该酶通过催化氨基水解反应表现出肽键形成活性。该酶利用D-Pro衍生物作为酰基供体底物，在一锅反应中高效合成了具有几丁质酶抑制活性的环（D-Pro- l - arg）。此外，我们从突变分析中鉴定出与酰基供体侧链识别相关的残基Asn193。

Peptides incorporating D-amino acids are expected to increase the functional variety and applications of peptides. In this study, we constructed the mutant D-aminopeptidases as a convenient tool for synthesis of peptides incorporating D-amino acids. We obtained a serine D-stereospecific amidohydrolase that exhibit peptide bond formation activity by catalysis of aminolysis reaction. The enzyme utilizes D-Pro derivatives as acyl donor substrate, and exhibit efficient synthesis of cyclo (D-Pro-L-Arg) that has chitinase inhibitory activity in a one-pot reaction manner. In addition, we identified the residue, Asn193, which associates the recognition of side chain of acyl donor, from the mutation analysis.