Development of the nucleoside analogs to harmonize with in vivo molecular function

本研究设计并合成了与双链DNA相互作用的核苷类似物。此外，我们的目标是建立使用这些核苷类似物调控基因表达的基础技术。我们发现，含有核苷类似物的寡核苷酸与双链体DNA相互作用，形成稳定的三链体DNA。并且该寡核苷酸抑制了培养细胞中的基因表达。

In this study, we designed and synthesized the nucleoside analogs which interact with duplex DNA. Moreover, we aimed to establish the basic technology of the regulation of the gene expression using these nucleoside analogs. We have found that oligonucleotide containing the nucleoside analogs formed the stable triplex DNA as a result of interaction between the nucleoside analogs and duplex DNA. And this oligonucleotide inhibited the gene expression in the cultured cell.