The mechanism of dephosphorylation of CERT by the reduction of raftcomponents

已知神经酰胺转运蛋白(CERT)是由质膜鞘磷脂的降解或神经鞘磷脂合成的抑制剂处理而触发的去磷酸化。我们的研究阐明了这两种去磷酸化途径在本质上是不同的。此外，意外地发现了CERT的一个新的磷酸化位点，该位点的磷酸化对先前报道的CERT的多重磷酸化有显著影响。结果表明，CERT的活性受更复杂的、多步骤的机制调控。

It is known that CERT (ceramide transport protein) is activated by the dephosphorylation, which is triggered by the degradation of plasma membrane sphingomyelin or by the treatment of inhibitor for sphoingomyelin synthesis. Our research elucidated these two dephosphorylation pathway is qualitatively different. Additionally, a novel phosphorylation site of CERT was found unexpectedly, and the phosphorylation of this site had a significant effect on the previously reported multiple phosphorylation of CERT. The results suggest that the activity of CERT is regulated by more complicated, multi-step mechanism.