Study of side effect reduction of anticancer agents by regulation of exogenous gene expression

为了在基因治疗中对基因导入后的转基因表达进行调控，分析了转基因细胞在抗癌药物作用下CMV启动子驱动的转基因表达水平。多柔比星(Dox)可使CMV启动子驱动的转基因表达水平升高。依达拉奉可降低Dox诱导的CMV启动子驱动的转基因表达。我们还发现，具有CMV启动子结合能力的AP-1的磷酸化途径被Dox激活。

For the purpose of regulation of transgene expression after gene introduction in gene therapy, the levels of CMV-promoter-driven transgene expression was analyzed when transgene introduced cells was exposed by anticancer agents. The levels of CMV-promoter-driven transgene expression were increased by doxorubicin (Dox) exposure. The induction of CMV-promoter-driven transgene expression by Dox was decreased by co-treatment of edaravone. We also found that phosphorylation pathway of AP-1 having CMV-promoter binding ability was activated by Dox.