Functional analysis of novel lipid-metabolizing enzymes belonging to the NlpC/p60 family

我们研究了最初被鉴定为肿瘤抑制基因的HRASLS家族成员可能的脂质代谢活性。属于HRASLS家族成员的H-rev107、HRASLS2和TIG3具有以甘油磷脂为底物的脂代谢活性。聚合酶链式反应分析表明，H-rev107和TIG3在所有受试组织中均有表达，其中H-rev107在脂肪组织中的表达最高。另一方面，HRASLS2在肝脏和肾脏中的表达占主导地位，在其他组织中表达水平较低。

We investigated possible lipid-metabolizing activities of HRASLS family members which were originally identified as tumor suppressor genes. H-rev107, HRASLS2 and TIG3, which are included in the HRASLS family members, showed lipid-metabolizing activities using glycerophospholipids as substrate. PCR analysis revealed that H-rev107 and TIG3 are expressed in all the tissues examined, with the highest expression of H-rev107 in adipose tissue. On the other hand, the expression of HRASLS2 was dominant in liver and kidney with low levels in other tissues.