Analysis of L(3)MBT transgenic mice

研究表明，基因表达的表观遗传调控在细胞增殖、分化和维持干细胞多能性等多种生物学行为中起着重要作用。本研究以多梳蛋白群中的L(3)MBT为研究对象，通过对L(3)MBT缺陷小鼠的研究，了解L(3)MBT的生理功能及其功能障碍对小鼠的影响。针对常规L(3) mbt缺失小鼠在胚胎早期死亡的情况，制备了一种可诱导缺失该基因的条件靶向载体。获得了与载体同源重组的胚胎干细胞，获得了嵌合小鼠。

It has been shown that epigenetic regulation of gene expression plays important roles in a variety of biological actions, such as cell proliferation, differentiation as well as maintenance of the purlipotency of the stem cells. In this study, we focused on L(3)MBT, one of the polycomb group proteins, and tried to investigate L(3)MBT deficient mice to know the physiological function of L(3)MBT and influences caused by its dysfunction. As conventional L(3)MBT-null mice died in the early embryonic phase, a conditional targeting vector which can be used for inducible deletion of the gene were prepared. ES cells which performed homologous recombination with the vector were obtained, and the chimeric mice were generated from the ES cells.