The development of immune regulation by genetically modified ES-DC

近年来的研究表明，DC在生理上对免疫应答起着重要的正、负调节作用。对于DC的遗传修饰，我们使用先前建立的方法从小鼠ES细胞体外产生DC（ES-DC）。我们尝试1）通过将表达胰岛衍生抗原的ES-DC沿着免疫抑制分子转移到NOD小鼠中来预防糖尿病，和2）通过转移表达免疫抑制分子的ES-DC来延长胰岛同种异体移植物存活。这些策略也可用于治疗患有自身免疫性疾病的受试者和用于诱导移植耐受。

Recent studies showed that DCs physiologically play significant roles in regulating immune responses both positively and negatively. For genetic modification of DC, we used a previously established method to generate DC from mouse ES cells in vitro (ES-DC). We tried 1) the prevention of diabetes by transfer of ES-DC expressing islet-derived antigen along with immunosuppressive molecules in NOD mice, and 2) the prolongation of islet allograft survival by transfer of ES-DC expressing immunosuppressive molecules. These strategies may also be useful for the treatment of subjects with autoimmune diseases and for the induction of transplantation tolerance.