New treatment of peri-implantitis with ointment which combine the effects of anti-inflammation and bone regeneration.

我们尝试研制一种具有抗炎、促进骨再生作用的种植体周围炎的治疗药物。已经证明，广泛用于降低胆固醇的HMG-CoA还原酶抑制剂（“他汀类”）刺激骨形成。我们以前的研究表明，在种植体部位局部应用氟伐他汀可以在愈合的早期阶段增强钛种植体周围的骨生成。此外，据报道，他汀类药物具有抗炎作用，并且他汀类药物的经皮应用促进了大鼠模型中的骨体积（Gutierrez等人，2006）。本研究的目的是探讨他汀类软膏对种植体周围成骨的影响。建立了大鼠口腔种植模型。大鼠口腔种植体模型与种植体周围组织模型之间无差异，因为难以评价炎症。假设软膏基质的选择是将高密度的他汀类药物与每种软膏混合，并检查每种软膏的特性。通过测量吸光度来检查他汀的持续递送量。因此，白色凡士林具有大量的他汀类药物缓释作用。在动物实验中，该软膏具有较长的作用持续时间，但口服情况不佳，含他汀类药物的软膏无效果。因此，有必要进行复查，如用薄膜覆盖等，以防止药膏在口腔中涂抹后唾液流出。

We try to make the ointment which had anti-inflammation and bone reproduction as a treatment for peri-implantites. It has been demonstrated that HMG-CoA reductase inhibitors ("statins"), which are widely used for cholesterol lowering, stimulates bone formation. And we previously showed that local application of fluvastatin at the implant site enhanced the osteogenesis around the titanium implant in early stages of healing. In addition, it has been reported that statin has anti-inflammatory effect, and transdermal application of statin promoted bone volume in rats model (Gutierrez at al. 2006). The aim of the present study was to investigate the effects of statin-containing ointment on peri-implant osteogenesis. The implant model in the rat mouth was able to be established. There was no differences between implant model in the rat mouth and peri-implantites model, because it was difficult to evaluate the inflammation. The selection of the ointment base was assumed to mix a high density of Statin with each ointment, and to examine the characteristic of each ointment. The amount of the sustained delivery of the Statin was examined by measuring the absorbance. As a results, white vaseline has a lots of sustained-relese of statin. It is ideal that the ointment have a longer duration of action on the animal experiment, but the condition of ointment in oral are not good and the statin-containing ointment have no effect. Therefore, it is necessary that the reexamination such as covering with the film etc. to prevent the outflow with saliva after the ointment is spread in the mouth.