The specific molecular mechanism of periodontal ligament fibroblast in periodontal ligament during tooth eruption

本研究旨在探讨牙周膜形成和维持的部分分子调控机制。本研究以萌出期牙周膜组织标本和萌出牙牙周膜组织标本为研究对象，探讨了在牙周膜组织中表达水平有显著差异的基因。本研究通过对牙周膜形成和调控相关基因的体内外研究发现，纤维调节素等基因在萌出鼠磨牙牙周组织和正常鼠磨牙牙周组织中的mRNA表达水平存在差异。然而，我们认为我们需要进一步研究方法的装置和对其他基因的研究，因为很难在每个细胞系中获得研究的再现性。

The aim of this study is to investigate a part of molecular regulatory mechanism of formation and maintenance of the periodontal ligament. I used the tissue sample of periodontal ligament during eruption and the periodontal ligament of the erupted tooth to investigate the genes which has a remarkable difference of the expression level, cyclopedically. I investigated the functions and the expression patterns of the genes likely to regulate formation and regulation of periodontal ligament in vivo and in vitro.Fibromodulin and some other genes presented different mRNA expression level between the periodontal tissue of erupting murine molar and the periodontal tissue of the erupted murine molar. However, it was thought that we required the device of the further investigation method and the investigation about other genes because it was difficult to get reproducibility of the investigation in each cell line.