Functional analysis of amelotin and its application for periodontal regeneration.

小鼠amelotin基因在小鼠成骨细胞株中过表达，诱导成骨细胞分化。我们观察到矿化结节的形成增强，而成骨细胞分化标记基因的表达没有改变。在免疫电镜观察中，我们还发现当细胞受到刺激时，淀粉蛋白有与钙和磷酸盐结合形成矿化结节的倾向。这些数据提示了阿米洛素促进骨形成的能力和应用于牙周再生的可能性。

Mouse amelotin gene was over-expressed in mouse osteoblatsic cell line and osteoblastic differentiation was induced. We observed enhanced mineralized nodule formation, whereas osteoblastic differentiation marker gene expressions were not changed. In immuno-electron microscopic observation, we also found the tendency of amelotin to bind to calcium and phosphate when the cells were stimulated to form mineralized nodule. These data suggested the capacity of amelotin to enhance bone formation and possibility of its application for periodontal regeneration.