Molecular mechanism of epithelialization by non-peptide, endogenous siderophores

中性粒细胞明胶酶相关脂质运载蛋白（Ngal或LCN 2）具有肾保护活性，肠螯合素（一种E.大肠杆菌来源的铁载体[Mori et al. J Clin Invest 2005]。哺乳动物铁载体的纯化导致邻苯二酚及其衍生物的鉴定[Bao et al. Nat Chem Biol 2010]。儿茶酚在很大程度上影响铁在哺乳动物中的组织分布。Ngal在肾缺血-再灌注损伤中发挥肾保护作用，而在3/4肾次全切除术中增强肾肥大和肾损伤[Viau et al. J Clin Invest 2005]。通过肾脏合成、中性粒细胞释放和近端小管重吸收受损确定尿Ngal水平[Kuwabara et al. Kidney Int 2009]。尿Ngal可用于评价慢性肾脏疾病的疾病严重程度和治疗疗效[Kasahara et al. Nephrol Dial Trasplant 2009]。Ngal报告小鼠允许Ngal表达的时空调节及其通过处理试剂的变化的实时可视化[Paragas et al. Nat Med 2011]。

Neutrophil gelatinase-associated lipocalin (Ngal or LCN2) has a renoprotective activity, which is enhanced by the presence of enterochelin, an E. coli-derived siderophore [Mori et al. J Clin Invest 2005]. Purification of mammalian siderophores lead to identification of catechol and its derivatives [Bao et al. Nat Chem Biol 2010]. Catechol largely affects tissue distribution of iron in mammals. Ngal plays a renoprotective role in renal ischemia-reperusion injury, whereas enhances renal hypertrophy and kidney injury in 3/4 subtotal nephrectomy [Viau et al. J Clin Invest 2005]. Urinary Ngal levels are determined by renal synthesis, release from neutrophils, and impaired reabsorption at proximal tubules [Kuwabara et al. Kidney Int 2009]. Urinary Ngal is useful for the evaluation of disease severity and treatment efficacy of chronic kidney disease [Kasahara et al. Nephrol Dial Trasplant 2009]. Ngal reporter mice allow real-time visualization of spaciotemporal regulation of Ngal expression, and its changes by treating reagents [Paragas et al. Nat Med 2011].