Screening for carcinogenesis-related proteins and diagnostic markers of pancreatic cancer by proteomic analysis

胰腺癌（PC）是最致命的恶性肿瘤之一，因为诊断晚，对各种治疗缺乏反应。为了从早期PC患者的血清中鉴定潜在的生物标志物，我们进行了双向凝胶电泳（2-DE）比较45例PC患者和20名健康志愿者的血清蛋白质组学谱。2-DE凝胶电泳显示2个差异表达蛋白点，经鉴定为α-1-抗胰蛋白酶（AAT）。这些蛋白质点也通过Western blotting证实。血清AAT亚型对PC的诊断和监测有一定的临床意义。

Pancreatic cancer (PC) is one of the most lethal malignant tumors because of late diagnosis and the lack of response to various therapies. To identify potential biomarkers in cancerous serum from early stage PC patients, we carried out two-dimensional gel electrophoresis (2-DE) to compare the serum proteomic profiles from 45 patients with PC and 20 healthy volunteers. Two spots showed differential expression on 2-DE gels and these up-regulated protein spots were identified as α-1-antitrypsin (AAT). These protein spots were also confirmed by Western blotting. The serum isoforms of AAT might be clinically useful for PC diagnosis and monitoring.