Development of hybrit mouse with humanized liver using superfreezing preserved human small hepatocytes.

在细胞移植治疗中，通过外科手术获取细胞供体是非常重要的。传统的肝切除术已被认为难以降低高发病率和死亡率。生理盐水联结电灼术能有效地封闭血管，减少出血。此外，这组患者术后Apo-B的恢复比传统的肝切除要快得多(肝胃肠病学2008；55：2188-2192)。PDX-1基因转导可将小肝细胞转化为胰岛素、胰高血糖素和生长抑素分泌细胞。此外，在转导PDX-1基因后，小肝细胞倾向于反式分化为β细胞，成熟肝细胞倾向于反式分化为α细胞(《肝胆胰脏外科杂志》2008；15：403-409)。另一方面，尿胰酶抑制物(UTI)是肝脏自身能产生的蛋白质之一。UTI缺乏的动物的肝脏再生受损(liver Int 2009；29：979-987.)。基因的表达可能取决于宿主条件和人类种族。因此，我们使用微DNA阵列分析比较了日本患者和芬兰患者的基因表达(基因染色体癌症2010；49：28-39。)我们对上述研究结果进行了验证，并在国内和国际会议上提交了数据。我们还相互讨论，以促进未来的进一步合作。

It is very important to perform surgical operation to obtain cell donation for cell transplantation therapy. Conventional hepatectomy has been considered to be difficult to decrease high mobidity with mortlity. A saline-linked electiric cautery was very effective to achieve vessel sealing and decrease bleeding. In addition, recovery of postoperative Apo-B in this group was much faster than conventional hepatectomy (Hepatogastroenterology 2008 ; 55 : 2188-2192.). Gene transduction of Pdx-1 could transform small hepatocyte into insulin, glucagon, and somatostatine producing cells. Furthermore, small hepatocytes tend to be trans-differentiated into beta cells and mature hepatocytes tend to be trans-differentiated into alpha cells after transduction of Pdx-1 gene (J Hepatobiliary Pancreat Surg 2008 ; 15 : 403-409.). On the other hand, urinary trypsin inhibitor (UTI) is one of the proteins which the liver could produce by itself exclusively. The liver regeneration has been impaired in UTI deficient animals (Liver Int 2009 ; 29 : 979-987.). The gene expression could be depending on host conditions and human races. So, we compared gene expressions using micro DNA array analysis among Japanese patients and Finn patients (Genes Chromosomes Cancer 2010; 49: 28-39.). We proved all above research results and presented the data in both national and international congresses. We also discuss each other to promote further collaboration in future.