Establishment of rhodopsin transgenic rabbit line and analysis of retinal degeneration

我们利用细菌人工染色体（BAC）转基因技术建立了视网膜色素变性（RP）的兔模型--视紫红质P347 L转基因（Tg）兔。这些兔子表现出视杆细胞占优势的进行性视网膜变性，光感受器的丧失具有明显的区域变化。Tg兔视网膜电图各成分均进行性下降，a波受影响程度大于b波，振荡电位（OPs）保存最好。有趣的是，年轻的Tg兔的OP振幅显着大于野生型兔。药理学实验表明，在年轻的Tg兔的显着较大的OP导致继发性改变的内部视网膜功能。

We have generated a rabbit model of retinitis pigmentosa (RP), the rhodopsin P347L transgenic (Tg) rabbit, by using bacterial artificial chromosome (BAC) transgenesis. These rabbits showed a rod-dominant, progressive retinal degeneration with a marked regional variations in the loss of photoreceptors. All of the electroretinogram components of the Tg rabbits decrease progressively with the a-wave more affected than the b-wave, and the oscillatory potentials (OPs) were the best preserved. Interestingly, the OP amplitudes of young Tg rabbits were significantly larger than those of wild-type rabbits. Pharmacological experiments showed that the significantly larger OPs in young Tg rabbits resulted from secondary alterations in the inner retinal function.