Immunolocalization of hormone receptors and their regulatory system in the hormonally responsive ducts of the mouse salivary glands

在反复注射雄激素和甲状腺激素后，在小鼠腮腺(PAG)的纹状管(SDS)中诱导出颌下腺(SMG)内的颗粒曲管(GCT)样细胞。这一发现和我们的系列研究表明，激素依赖的GCT是小鼠三大唾液腺的共同特征。然而，从未成熟的颗粒细胞到GCT细胞的细胞分化过程在每个腺体中都会有所不同。这一过程是在SMG中的大量SD细胞中完成的，其中一小部分局限于舌下腺(SLG)，但所有SD细胞在PAG中都保持其原始形态。腺特异性GCT表型可能与激素受体的含量有关。然而，我们已经尝试使用几种商用的雄激素受体抗体来检测小鼠唾液腺中雄激素受体(AR)的免疫定位和蛋白质印迹，但未获成功。可想而知，唾液腺和生殖器官中ARs的分子结构可能不同。

Granular convoluted tubule (GCT) -like cells in the submandibular gland (SMG), were induced in the striated ducts (SDs) of the parotid gland (PAG) of mouse after the repeatedly injections of androgen and thyroid hormone. This finding and our serial studies suggest that the hormone-dependent GCT is a common characterization in murine three major salivary glands. However, the process of cytodifferentiation from immature granular cells to GCT cells will be varying in each gland. This process is completed in a large population of SD cells in the SMG, with a small population restricted to the sublingual gland (SLG), but all of the SD cells maintain their original form in the PAG. The gland-specific GCT-phenotype might be caused by contents of hormone receptors. However, we have tried unsuccessfully to detect immunolocalization and western blotting of androgen receptor (AR) in the mouse salivary glands, using several commercially available antibodies for ARs. It is conceivable that molecular structures of ARs may be different between the salivary glands and the reproductive organs.