Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy

新的感染根管菌群分析方法的建立及其在根管治疗中的应用本研究的目的是利用厌氧培养和分子生物学技术对根管治疗前后感染根管中的菌群进行分析。治疗前后分别采集感染根管标本，进行厌氧培养。培养结束后计数CFU数，16S rRNA基因测序鉴定分离菌。这项研究的结果表明，根管内的环境是厌氧的，因此支持厌氧菌的生长，而适当的治疗会极大地改变根管环境。

Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy The aim of this study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacterial identification, before and after root canal treatment. Samples from infected root canals before and after treatment were collected, followed by anaerobic culture. After incubation, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. The findings of this study suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.